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Amerigo Scientific Launches PNGase F for Glycan Release
October 22, 2021
Amerigo Scientific, a distribution company focused on providing critical products and services to the biomedical and life science research communities, now introduces PNGase F for life science researchers, which is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing a(1-3) linked core fucose commonly found on plant glycoproteins.
PNGase F (Peptide N Glycosidase F) cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins. The enzyme deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. The enzyme will remain fully active under reaction conditions (37°C) for at least 96 hours. PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoprotein.
There are a number of alternative enzymes which can be used to remove N-glycans, most especially the Endo F family of enzymes and Endo H. These enzymes cleave between the two N-acetylglucosamine residues in the core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. This leaves a charged sugar which can assist in keeping proteins in solution that precipitate after deglycosylation with PNGase F which removes the oligosaccharide intact. Endo F1 cleaves high mannose and some hybrid type N-glycans. Endo F2 removes biantennary and high mannose (at a 40X reduced rate). Endo F3 releases triantennarry and fucosylated biantennary N-glycans. Endo H removes hybrid or high mannose glycans.
Amerigo Scientific offers a comprehensive collection of enzymes from global manufacturers covering all the core biological research areas. Enzymes are biological catalysts that regulate the rate of chemical reactions that proceed in organisms and play irreplaceable roles in signal transduction pathways and cell regulation pathways. And this newly released PNGase F is an extension of Amerigo Scientific's enzymes product portfolio.
"We hope this PNGase F released for the scientific research community can help enable our customers to continue their life science studies and advance their research pace. For any of customers' specific requirements, we ensure to provide them with cutting-edge solutions, products and efficient services. And we are proud to have a variety of talented and dedicated employees to help our customers to achieve their goals and gain more during the research process." said Nina Cooper, Ph.D., the chief scientist at Amerigo Scientific.
For more information about the PNGase F, please visit https://www.amerigoscientific.com.
About Amerigo Scientific
Amerigo Scientific is a distribution company that focuses on providing critical products and services to the biomedical and life science research communities. Although it is a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in top international pharmaceutical and biotech companies, academic research institutes, and government research agencies. It has a professional team, as most of its employees own a graduate (Ph.D. or master) degree in life science, so they can understand customers' questions or concerns and are always ready to provide individualized customer service with high standard.
PNGase F (Peptide N Glycosidase F) cleaves N-linked (asparagine-linked) oligosaccharides from glycoproteins. The enzyme deaminates asparagine to aspartic acid, leaving the oligosaccharides intact. Denaturation increases the rate of cleavage. Most native proteins can still be completely N-deglycosylated but incubation time must be increased. The enzyme will remain fully active under reaction conditions (37°C) for at least 96 hours. PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoprotein.
There are a number of alternative enzymes which can be used to remove N-glycans, most especially the Endo F family of enzymes and Endo H. These enzymes cleave between the two N-acetylglucosamine residues in the core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. This leaves a charged sugar which can assist in keeping proteins in solution that precipitate after deglycosylation with PNGase F which removes the oligosaccharide intact. Endo F1 cleaves high mannose and some hybrid type N-glycans. Endo F2 removes biantennary and high mannose (at a 40X reduced rate). Endo F3 releases triantennarry and fucosylated biantennary N-glycans. Endo H removes hybrid or high mannose glycans.
Amerigo Scientific offers a comprehensive collection of enzymes from global manufacturers covering all the core biological research areas. Enzymes are biological catalysts that regulate the rate of chemical reactions that proceed in organisms and play irreplaceable roles in signal transduction pathways and cell regulation pathways. And this newly released PNGase F is an extension of Amerigo Scientific's enzymes product portfolio.
"We hope this PNGase F released for the scientific research community can help enable our customers to continue their life science studies and advance their research pace. For any of customers' specific requirements, we ensure to provide them with cutting-edge solutions, products and efficient services. And we are proud to have a variety of talented and dedicated employees to help our customers to achieve their goals and gain more during the research process." said Nina Cooper, Ph.D., the chief scientist at Amerigo Scientific.
For more information about the PNGase F, please visit https://www.amerigoscientific.com.
About Amerigo Scientific
Amerigo Scientific is a distribution company that focuses on providing critical products and services to the biomedical and life science research communities. Although it is a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in top international pharmaceutical and biotech companies, academic research institutes, and government research agencies. It has a professional team, as most of its employees own a graduate (Ph.D. or master) degree in life science, so they can understand customers' questions or concerns and are always ready to provide individualized customer service with high standard.