Creative Biolabs Illustrated Its Newly-developed Antibody Affinity Maturation Service

SHIRLEY, NY - Sep 18, 2018 - Sep 18th, 2018, Creative Biolabs, as one of the most experienced supplier in various antibody production and engineering fields, has recently released its newly-developed affinity maturation antibody, which is an indispensable process to improve antibody affinity for an antigen.

It is recognized that there are two main systems that affinity maturation happens: in vivo and in vitro.

In vivo, the affinity maturation occurred in the germinal centers of the secondary lymphoid organs. And there are two interrelated processes included---clonal selection and somatic hypermutation. In vitro, affinity maturation is determined by the mutation and selection. And the in vitro affinity maturation has been successfully and widely used to optimize antibodies, antibody fragments as well as other peptide molecules, such as antibody mimetics.

In creative Biolabs, scientists are gained extensive experience in antibody affinity maturation. And in this process, scFv is always as the antibody format. Additionally, a monovalent display phagedmid system is also employed to reduce the avidity effects during antigen-binding screening. What’s more, affinity maturation services for single domain antibodies are available.

The main and popular methods in Creative Biolabs are oligonucleotide-directed mutagenesis and untargeted mutagenesis, which allow scientists to process construct random or defined sublibraries.

Oligonucleotide-directed mutagenesis

It is known that some antibody or antigen positions cannot be randomized at a defined diversity to improve the affinity till the structure of the antibody or antigen is available or modeling the structure is possible. It is notable that Creative Biolabs is capable of creating any sublibraries to incorporate the defined mutations with the assistance of trimer codon technology. It is very confident for scientists in Creative Biolabs to supply antibody affinity maturation due to the frequent study of AA sequences of the antibody to find out the conserved sequences.

Untargeted mutagenesis

Suring sub-library construction, scientists in Creative Biolabs always employe an error-prone PCR integrated DNA-shuffling method to mutate mainly CDR regions. If it is possible to introduce immunogenic mutations to framework positions, scientists usually use this approach to create mutations at completely random positions across the entire VH and VL fragments.

Other than these, some services involved in this part are also available in Creative Biolabs, such as antibody affinity measurement and peptide affinity maturation.

About Creative Biolabs

With more than ten-year experience in antibody production field, Creative Biolabs has supplied services and production for both industrial use and academia use. As affinity maturation is very important in antibody production, Creative Biolabs illustrated antibody affinity maturation services to assist researchers.