What Can We Benefit from MemProArt™ Platform

SHIRLEY, NY, US - Mar 14, 2016 - Membrane proteins consist of 20~30% of all encoding genes in genome. They participate in various biological processes, such as ligand-receptor binding, molecule transportation, signal transduction, intercellular recognition and enzymolysis.

Growing interest is focusing on their potential in antibody-based therapy and diagnosis for various diseases. However, the traditional antibody preparation come across drawbacks in the expression of membrane protein which serves as the antigen, which include low expression level, difficult extraction, heterogeneous production, misfolding, incomplete modification and conformational change. These drawbacks directly lead to low efficiency of corresponding antibody production, and hinder the progress of research project.

We have already known that research methods and professional practices of membrane protein antibody are constantly changing recent years. The market trend proves that only by acquiring the latest techniques timely can we get ahead in this field and obtain remarkable success.

Many companies and institutions are taking actions on that. Backed up by years of experience in membrane protein and antibody research, Creative Biolabs successfully developed world-leading production service of antibody targeting membrane protein.

They have developed MemProArt™ platform to meet the increasing needs from the market. The principle is to directly express and present target antigen of native conformation on the cell membrane, proteoliposome or lipoparticles. Obtaining immunoreactivity from membrane protein with native conformation, this platform has conquered the shortcomings of traditional methods and has been proved to be of high efficiency in generating hundreds of antibodies. 

The MemProArt™ platform provides three independent methods for membrane protein antigen expression.

Cell-based Expression

This is a break-through technology aiming the maximum preservation of the native conformation of membrane protein epitopes, allowing high efficient antibody production. 

Cell-free Expression

This is derived from a unique cell-free expression system, enables membrane proteins directly incorporated into artificial liposomes (both bilayer and planar vehicles) during the in vitro translation step, resulting in “ready-to-use” proteoliposome for immunization.

Virus-like Particles (VLPs) and Lipoparticles

This resembles the self-assembly of virus and produces protein in correct conformation. Amplifying retroviral (HIV-1 or MLV) gag protein in 293 or CHO-based EBEE™ technique, our patent technique is able to introduce adequate posttranslational modification pattern in the expressed membrane protein so as to achieve the highest immunogenicity.

In addition to these three independent methods for membrane protein antigen expression, the MemProArt™ platform has also developed phage display library screening and hybridoma technique for monoclonal antibody screening and production respectively.

About Creative Biolabs

Creative Biolabs is specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs of early antibody drug discovery and development.