CD Genomics Announces Highly Sensitive and Accurate Genome Sequencing Services Related to Genome Research

SHIRLEY, NY - Jun 24, 2019 - SHIRLEY, NY, UNITED STATES - Jun 24, 2019 - CD Genomics, the USA-based genetic sequencing company and an expert in transcriptomics and next generation sequencing, announces the technical escalation of sequencing services, The updated service ranges include five: mitochondrial DNA sequencing, microbial whole genome sequencing, plasmid DNA sequencing, SMRT sequencing and bacterial genome sequencing, which are based on some of the most powerful platforms in this field with unrivaled accuracy and sensitivity.

Mitochondria play a very important role in important cellular functions. In addition to producing more than 90% of energy need by the cells, mitochondria also produce reactive oxygen species (ROS) and are involved in apoptosis and other important cellular functions. Mutant mtDNA and wild type can coexist as heterogeneity and cause human diseases including cancer, heart disease, diabetes, Alzheimer's disease, Parkinson's disease and hypertension. Because of the considerable clinical variability between mitochondrial diseases and many patients who exhibit phenotypes that overlap with disease, pathogenic mtDNA variants can usually only be identified by molecular genetic testing of DNA extracted from blood samples to confirm the diagnosis. Mitochondrial DNA sequencing is a useful tool for studying human disease research and can also be used for population genetics and biodiversity assessment. By utilizing PacBio's long reads, CD Genomics provides non-amplified, full-length sequencing of linearized mtDNA. Full-length sequencing allows for variable phasing along the entire mitochondrial genome, identification of heterogeneous variants, and detection of epigenetic modifications lost in the amplicon-based methods.

Microbial whole genome sequencing produces a large amount of data that enables a comprehensive assessment of all genetic characteristics of isolated microorganisms. Microbial whole genome sequencing is critical for accurate microbial identification, complete reference genome generation (de novo sequencing), comparative genomic studies (re-sequencing) and genome development. Comparative genomic studies can identify individual genetic variation and large-scale structural variation within a population of reference genomes available. Therefore, evolutionary features and phylogenetic relationships can be inferred. Microbial whole genome sequencing provides the possibility of gene discovery and annotation. After explaining multiple genes, new biochemical pathways that may be beneficial to medicine and biotechnology may be identified.

Plasmids are thought to play an important evolutionary role in microbial communities by laterally introducing genes and traits into microbial genomes. Plasmids are important carriers for bacterial populations to rapidly adapt to changing environmental conditions. The genetic variation produced by the plasmids carried within the population ensures robustness to environmental changes. Plasmid-mediated gene transfer plays an important role not only in the mobilization and spread of antibiotic resistance genes, but also in the pathogenesis of pathogens and the transmission of pathogenic determinants. Plasmid DNA sequencing is rapidly becoming the standard method to increase our understanding of the genetic diversity and evolutionary history of plasmids.

The highly repetitive elements found in both eukaryotic and prokaryotic genomes pose challenges to genome assembly and make detailed studies of repetitive sequences difficult. Long read sequences can provide reads in excess of a few or tens of kilobases (kbs), which can span complex or repetitive unamplified molecules as described above, and can also exhibit positions where methylated bases occur, such provide functional information of DNA methyltransferase encoded by methylated transcriptase. PacBio SMRT sequencing has unique advantages in the studies of de novo genomics, metagenomics, transcriptomics and epigenetics.

It is well known that the repetitive stretches of DNA are abundant and are one of the main technical challenges that hinder accurate sequencing and genome assembly work. In the case of bacteria, the rRNA gene operon is usually the largest region of the repeat sequence, ranging in size from 5 to 7 kb. Microbial whole-genome sequencing of the Illumina HiSeq platform uses sequencing by synthesis technology, which is limited by read length, currently ranging from 50 to 300 bp, and due to the need for PCR amplification of multiple DNA templates prior to sequencing, It is possible to perform base composition which may affect the G + C content of the sequence. CD genomics is providing PacBio sequencing to increase bacterial genome sequencing research methods. A comprehensive understanding of bacterial genomes, including genes, regulatory regions, IS components, phage integration sites, and base modifications is critical to understanding key features such as antibiotic resistance, virulence, and metabolism.

“CD Genomics concentrates on supplying the high-quality services and products for labs and research institutes. We guarantee the developed products and services, which are customized, can meet the demands of our clients, who believe in our scientists and CD Genomics”, said Dr. David.

About CD Genomics

CD Genomics is a world-leading genomics services company that innovates sequencing services, as well as genotyping, library construction, bioinformatics, aptamers, microarray, health diagnostics, mutagenesis analyses and others for the genomics industry.

Contact

Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Email: contact@cd-genomics.com