New 2D Superresolution mode for ZEISS Airyscan delivers 120 nanometer lateral resolution
BOSTON, MA, UNITED STATES - Apr 17, 2018 - New 2D Superresolution mode for ZEISS Airyscan delivers 120 nanometer lateral resolution
Improved optical sectioning delivers higher resolution without the need to acquire a z-stack
JENA/Germany
At Neuroscience 2017, a new imaging mode for the ZEISS LSM 8 family with Airyscan has been introduced. Their unique 32-channel GaAsP array detector captures more spatial information than traditional confocal microscopes. The new 2D Superresolution mode now uses this additional information to create an optical section of 0.2 Airy units (AU) and resolves structures down to 120 nanometer laterally in a single image.
The benefits for scientists
In the past, researchers had to acquire a stack of z-slices and subsequently deconvolve to get optical sections thinner than one AU and enhance lateral resolution. Temporal resolution was thus limited, and a prolonged light exposure of the sample was inevitable. Scientists can now use the new 2D Superresolution mode to overcome this problem and perform gentle live cell imaging experiments. They profit from very low light exposure, highly resolved structural information and excellent signal-to-noise ratio.
The principle behind
ZEISS Airyscan is an area detector. Unlike traditional confocal microscopes which reject photons from outside of the focal plane at a pinhole, ZEISS Airyscan detects all precious fluorescence emission photons of 1.25 AU. Their information is then used to deliver higher sensitivity, superresolution, and high acquisition speeds. The new 2D Superresolution mode takes advantage of the fact that ZEISS Airyscan captures x, y and z information of the confocal point spread function. A new exclusive processing algorithm uses this inherent spatial information captured in a single image. It specifically distinguishes between photons originating from the focal plane of 0.2 AU and photons from outside of this focal plane. In a traditional confocal microscope, a researcher could only close the pinhole to 0.2 AU to attempt to achieve the same optical sectioning. This would mean sacrificing many photons, even from the focal plane, thus reducing signal-to-noise drastically.
Researchers can process both existing and new ZEISS Airyscan data with the new 2D Superresolution mode.
About ZEISS
ZEISS is an internationally leading technology enterprise operating in the optics and optoelectronics industries. The ZEISS Group develops and distributes semiconductor manufacturing equipment, measuring technology, microscopes, medical technology, eyeglass lenses, camera and cine lenses, binoculars and planetarium technology. With its solutions, the company constantly advances the world of optics and helps shape technological progress. ZEISS is divided up into the four segments Semiconductor Manufacturing Technology, Research & Quality Technology, Medical Technology, and Vision Care/Consumer Optics. ZEISS is represented in over 40 countries and operates more than 30 production sites, over 50 sales and service locations and about 25 research and development facilities.
In fiscal year 2014/15 the company generated revenue approximating €4.5 billion with around 25,000 employees. Founded in 1846 in Jena, the company is headquartered in Oberkochen, Germany. Carl Zeiss AG is the strategic management holding company that manages the ZEISS Group. The company is wholly owned by the Carl Zeiss Stiftung (Carl Zeiss Foundation).
Further information at www.zeiss.com